Avian Influenza Sampling Procedures & Lab Testing [FAO videos]

Repost

– Gameness til the End

Avian Influenza sampling procedures and laboratory testing

Funded by FAO and the Istituto Zooprofilattico delle Venezie (IZSVe)

• Welcome – 2 videos
• Diagnostic Sample – 4 videos
• Serological Tests – 3 videos
• Molecular Tests – 5 videos

1 WELCOME

1.1 Welcome address by Juan Lubroth

Chief Veterinary Officer of the Animal Production and Health Division at the FAO of the United Nations

1.2 Welcome address by Ilaria Capua

Director of the OIE/FAO Reference Laboratory for Avian Influenza and Newcastle disease

2 AVIAN INFLUENZA DIAGNOSTIC SAMPLE

2.1 Requirements for shipment of avian influenza suspected samples

2.2 Necropsy

2.3 Sample collection in live birds: blood, tracheal and cloacal swat

2.4 Personal Protective Equipment (PPE)

3 AVIAN INFLUENZA SEROLOGICAL TESTS

3.1 Agar gel immunodiffusion assay (AGID)

3.2 Enzyme Linked Immunosorbent assay. Competitive and Indirect (ELISA)

3.3 Haemagglutination inhibition test (HI)

4 AVIAN INFLUENZA MOLECULAR TESTS

4.1 Preparation of the samples for RNA extraction

4.2 Result visualization by Agarose Gel electrophoresis

4.3 Real-time PCR

4.4 PCR Master Mix preparation and RT-PCR

4.5 Total RNA extraction