Have you considered using artificial insemination and battery cages together as a gamecock breeding system?
One brood cock for ten brood hens? One brood cock for fifty brood hens?
Our previous articles with Artificial Insemination for chickens and poultry:
- How To Breed A Gamecock That Is Blind and Crippled Both Feet?
- How To Breed A Gamecock That Is Dead Game?
- Genetic Engineering and Cloning in Poultry Industry 2003
Let us watch and read more about Artificial Insemination for chickens and poultry in the videos and the articles below to learn its benefits and faults.
– Gameness til the End
Articles on Artificial Insemination for Chickens and Poultry
Story by Sophie miyumo (research assistant at InCIP-research unit, Egerton University)
Artificial insemination is the most widely used reproductive technology in the livestock industry. Its adoption in poultry species has increased in popularity, especially in the western countries for research and commercial purposes. In Kenya, this technique is mostly used by poultry research and breeding centres. However, there are scenarios in which commercial poultry farmers may benefit from artificial insemination in poultry:
- In broilers, the males have extreme body conformation, broad bodies and short legs, which hinder natural mating. Also, as the selection for faster growth rates in broilers is intensified, fertility in males is likely to decline due to the negative relationship between growth and fertility. Application of AI in such scenarios is cost effective in broiler breeding management.
- AI allows for incompatible individuals to mate; incompatibility arises when males are heavier than females and under natural mating this may result to injury of the females.
- AI allows for better use of the cage feeding system in hatchery operations, especially when dealing with large number of females that are required to lay fertilized eggs.
- AI allows for one male of high genetic merit for a particular trait of interest to serve more females therefore, increases the number of offspring per cock compared to natural mating which is limited to a mating ratio of 1male:10 females.
Artificial insemination in chicken requires one to understand the basic anatomy and physiology of the hen’s and the cock’s reproductive tract. In addition to this one must be technically competent with the semen collection and deposition procedures in order to achieve effectiveness in producing fertilized eggs.
Semen collection procedures
Prior to semen collection, cocks need to be trained and this is achieved through abdominal and back massage for about a minute for 3 days, consecutively. The abdominal massage method is the most commonly used since it is non-invasive and has minimal stress on the cock. The procedure involves restraining the male, followed by gentle but rapid stroking of the abdomen and back region (testes are located in this region) towards the tail. This stimulates the copulatory organ causing it to protrude. At this point, the handler quickly pushes the tail forward with one hand and, at the same time, using the thumb and forefinger of the same hand to gently squeeze the region surrounding the sides of the cloaca to “milk” semen from the ducts of the copulatory organ. Semen may then be collected in a small tube or any cup-like container. This procedure is repeated twice, once a day; an additional round may cause damage to the testes and cloacal region.
The volume of semen that can be collected from a single cock ranges from about 0.7 to 1.0 ml, with a spermatozoon concentration of 3 to 4 billion/ml. However, the quantity of semen depends on genetics and environmental factors such as age, bodyweight, season and nutrition. The degree to which the male will respond to the abdominal massage technique and the pressure applied on the ejaculatory ducts will also influence the quantity of semen produced. Chicken semen begins to lose fertilizing ability when stored for more than 1 hour; therefore it must be deposited in the hen within the 1 hour of collection. In the case of short-term storage and transportation of the semen, it is necessary to use liquid cold (4⁰c) storage to maintain spermatozoa viability for up to 24 hours.
Semen deposition procedure
Vaginal insemination is commonly used for semen deposition as there are less risks of injury the hen. Preliminary stroking and massaging of the back and abdomen is required to stimulate the hen. This is followed by applying pressure to the left side of the hen’s abdomen around the vent causing evertion of the cloaca hence protrusion of the vaginal orifice. An inseminator containing the semen is inserted 2.5 cm deep into this opening for semen to be deposited. As the semen is expelled by the inseminator, pressure around the vent is released, so that the oviduct can return to its normal position and draw the semen inwards to the utero-vaginal junction. Inseminators such as straws, syringes or plastic tubes may be used. During insemination, the volume of semen required per hen is about 0.1ml which contains about 100 to 200 million sperms. Timing of the insemination should be considered. It is best to inseminate hens in the late afternoon (2:00pm and 4:00pm), since in the morning hours hens may have an egg in the oviduct, making it difficult for the sperm to swim up to the ovary. A significant feature of the reproductive physiology of the hen is her ability to store fertile spermatozoa for up to 14 days in the sperm storage tubules located at the utero-vaginal junction. The tubules release the semen, slowly over time, which swim to the fertilization site and therefore allows for hens to be inseminated consecutively for two days for the first time, and thereafter at regular intervals of 14 days. Twenty-four hours after insemination, egg-breakout analysis is carried out to determine egg fertility.
Currently, the Smallholder Indigenous Chicken Improvement Program (InCIP) – research unit at Egerton University offers training to interested farmers on the artificial insemination in poultry. The training does not require any background on poultry science, just an individual’s interest. This is because the training covers the fundamentals of the reproductive anatomy and physiology of the male and female, at a theoretical and practical level. Thereafter, the trainees are taken through a practical lesson on semen collection and deposition techniques, and egg fertility analysis. The training takes a period of two weeks and the expectation at the end of it is that individuals have the capacity to carry out semen collection from males (abdominal massage, semen milking and semen handling), semen deposition in females (cloacal evertion, semen deposition) and differentiate fertile eggs from infertile eggs.
By R. Keith Bramwell, BS, MS, PhD, University of Arkansas-Fayetteville
Artificial insemination (AI) is widely used to overcome low fertility in commercial turkeys, which results from unsuccessful mating as a consequence of large, heavily muscled birds being unable to physically complete the mating process. This is a serious and costly problem in the production of commercial turkey hatching eggs. In most commercial chicken production systems in the USA, it has not been necessary to implement AI programs because natural mating results in adequate fertility levels, but AI is routinely used in special breeding work and research. However, as managing commercial broiler breeders to maximize fertility becomes more challenging, the use of AI in commercial poultry operations outside the USA is becoming more common. Certainly, the use of AI in chickens, as in turkeys, can improve fertility; however, the cost of implementing AI on a large scale is often cost prohibitive.
Collecting semen from a chicken or turkey is done by stimulating the copulatory organ (the phallus) to protrude by massaging the abdomen and the back over the testes. This is followed quickly by pushing the tail forward with one hand and, at the same time, using the thumb and forefinger of the same hand to apply pressure in the area and to “milk” semen from the ducts of this organ. Semen flow response is quicker and easier to stimulate in chickens than in turkeys. The semen may be collected with an aspirator (turkeys) or in a small tube or any cup-like container. In turkeys, the volume averages ~0.35–0.5 mL, with a spermatozoon concentration of 6 to >8 billion/mL. In chickens, volume is 1–2 times that of turkeys, but the concentration is about one-half. Collected semen is usually pooled and diluted with an extender before use.
Chicken and turkey semen begins to lose fertilizing ability when stored >1 hr. Liquid cold (4°C) storage of turkey and chicken semen can be used to transport semen and maintain spermatozoal viability for ~6–12 hr. This short-term storage of semen is common in turkeys, while not as common in chickens. When using liquid cold storage for >1 hr, turkey semen must be diluted with a semen extender at least 1:1 and then agitated slowly (150 rpm) to facilitate oxygenation; chicken semen should be diluted and then cooled—agitation is not necessary. Chicken and turkey semen may be frozen, but reduced fertility limits usage to special breeding projects. Under experimental conditions, fertility levels of 90% have been obtained in hens inseminated at 3-day intervals with 400–500 million frozen-thawed chicken spermatozoa.
Several commercial semen extenders are available and are routinely used, particularly for turkeys. Extenders enable more precise control over inseminating dose and facilitate filling of tubes. Results may be comparable to those using undiluted semen when product directions are followed. Dilution should result in an insemination dose containing ~300 million viable spermatozoa for turkeys. However, the number of spermatozoa inseminated will range from 150–300 million viable cells depending on the age of the turkey hens inseminated. In chickens, the number of diluted semen inseminated will range from ~100–200 million sperm cells per insemination. Producers usually determine the spermatozoa concentration and dilute the semen to obtain the appropriate sperm cell concentration for either the turkey or chicken.
For insemination, when holding the hen upright, pressure is applied to the abdomen around the vent, particularly on the left side. This causes the cloaca to evert and the oviduct to protrude, so that a syringe or plastic straw can be inserted ~1 in. (2.5 cm) into the oviduct and the appropriate amount of semen delivered. As the semen is expelled by the inseminator, pressure around the vent is released, which assists the hen in retaining sperm in the vagina or oviduct. When inseminating undiluted turkey semen, the high sperm cell concentration allows for 0.025 mL (~2 billion spermatozoa) to be inseminated at regular intervals of 7–10 days, yielding optimal fertility. In chickens, because of the lower spermatozoon concentration and shorter duration of fertility, 0.05 mL of undiluted pooled semen, at intervals of 7 days, is required. The hen’s squatting behavior indicates receptivity and the time for the first insemination. For maximal fertility, inseminations may be started before the initial oviposition in turkeys, whereas this is not necessary in chickens. Fertility tends to decrease later in the season; therefore, it may be justified to inseminate more frequently or use more cells per insemination dose as hens age.
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